Western Blot: NUP155 Antibody [NBP2-22286] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. NBP2-22286 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: NUP155 Antibody [NBP2-22286] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22286 used for IP at 6 ug/mg lysate. NUP155 was also immunoprecipitated by rabbit anti-NUP155 antibody. For blotting immunoprecipitated NUP155, NBP2-22286 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.