Western Blot: TIP120B Antibody [NBP2-22334] - Samples: Whole cell lysate (50 ug) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: NBP2-22334 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: TIP120B Antibody [NBP2-22334] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22334 used for IP at 6 ug/mg lysate. TIP120B was also immunoprecipitated by rabbit anti-TIP120B antibody. For blotting immunoprecipitated TIP120B, NBP2-22334 was used at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.