Western Blot: taxilin gamma Antibody [NBP2-22331] - Whole cell lysate (50 ug) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Gamma-Taxilin antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunoprecipitation: taxilin gamma Antibody [NBP2-22331] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22331 used for IP at 6 ug/mg lysate. Gamma-Taxilin was also immunoprecipitated by rabbit anti-Gamma-Taxilin antibodies NBP2-22329 and NBP2-22330. For blotting immunoprecipitated Gamma-Taxilin, NBP2-22331 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.