Western Blot: GART Antibody [NBP2-32207] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-GART antibody NBP2-32207 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: GART Antibody [NBP2-32207] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- GART used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Immunoprecipitation: GART Antibody [NBP2-32207] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-GART antibody NBP2-32207 used for IP at 6 ug per reaction. For blotting immunoprecipitated GART, NBP2-32207 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.