Western Blot: SETD3 Antibody [NBP2-32136] - Samples: Whole cell lysate (50 ug) from Jurkat, HeLa, 293T, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-SETD3 antibody NBP2-32136 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 minute.
Immunoprecipitation: SETD3 Antibody [NBP2-32136] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SETD3 antibody NBP2-32136 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated SETD3 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.