Western Blot: UMPS Antibody [NBP2-32172] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-UMPS antibody NBP2-32172 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: UMPS Antibody [NBP2-32172] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-UMPS antibody NBP2-32172 used for IP at 6 ug per reaction. For blotting immunoprecipitated UMPS, NBP2-32172 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.