TBP (D5G7Y) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 12578
• Prices: $246.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D5G7Y
• Applications: WB IP ChIP
• Species Reactivities: Human, Monkey
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala110 of human TBP protein.
• Description: Rabbit Monoclonal
• Gene: TBP

Product images

Western blot analysis of extracts from various cell lines using TBP (D5G7Y) Rabbit mAb.

Immunoprecipitation of TBP from HCT 116 cell extracts, using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or TBP (D5G7Y) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using TBP (D5G7Y) Rabbit mAb.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HeLa cells and either 10 μl of TBP (D5G7Y) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human γ-Actin Promoter Primers #5037, SimpleChIP ® Human GAPDH Promoter Primers #4471 and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.