Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HCT 116 cells and either 10 μl of TCF4/TCF7L2 (C9B9) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human CaMK2D Intron 3 Primers #5111, human c-Myc promoter primers, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Western blot analysis of total cell lysates from HCT116, DLD1 and HepG2 cells using TCF4/TCF7L2 (C9B9) Rabbit mAb.