Western Blot: Albumin Antibody [NBP2-26510] - Lane 1: molecular weight. Lane 2: mouse serum albumin. Load: 0.1 ug. Primary antibody: MSA antibody at 1:1000 in 1% BSA-TTBS for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:20,000 for 45 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) 30 min at 4C. Predicted/Observed size: 69 kDa, 72 kDa for MSA. Other band(s): none.
Western Blot: Albumin Antibody [NBP2-26510] - Analysis using the Biotin conjugate of NBP2-26510. Detection of Lane 1: Human Serum Albumin. Load: 50 ng per lane. Used at a dilution of 1:1,000 overnight at 4C.
Western Blot: Albumin Antibody [NBP2-26510] - Analysis using the HRP conjugate of NBP2-26510. Detection of Lane 1: 1:400 of FBS. Lane 2: 1:1600 of FBS. Lane 3: 1:400 of normal rabbit serum. Lane 4: 1:1600 of normal rabbit serum. Lane 5: 1:400 of normal human serum. Lane 6: 1:1600 of normal human serum.
SDS-Page: Albumin Antibody [NBP2-26510] - Lane 1: mouse serum albumin reduced. Lane 2: molecular weight. Lane 3: mouse serum albumin non-reduced. Load: 1.0 ug. Predicted/Observed size: Analysis by SDS-PAGE shows a banding pattern consistent with purified Rabbit IgG. Other band(s): none.