Western blot analysis of extracts from THP-1 cells, differentiated with TPA #4174 (80 nM, overnight) and then untreated (-) or treated with LPS (1 μg/ml, 1 hr; +), using TNF-α (D1G2) Rabbit mAb (IF/Flow Preferred) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Confocal immunofluorescent analysis of THP-1 cells, treated with TPA #4174 (80 nM, 16 hr) (left) or treated with TPA followed by LPS (1 μg/ml, 1 hr) (right), using TNF-α (D1G2) Rabbit mAb (IF/Flow Preferred) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).
Flow cytometric analysis of human peripheral blood lymphocytes, untreated (left) or treated with TPA #4174 (40 nM, 4 hr), Ionomycin #9995 (2 μM, 4 hr), and Brefeldin A #9972 (1 μg/ml, last 3 hr of treatment) (right), using a CD3 antibody and TNF-α (D1G2) Rabbit mAb (IF/Flow Preferred).