Western Blot: B4GALT4 Antibody (5E2) [H00008702-M01] - Analysis of B4GALT4 expression in transfected 293T cell line by B4GALT4 monoclonal antibody (M01), clone 5E2.Lane 1: B4GALT4 transfected lysate(40 KDa).Lane 2: Non-transfected lysate.
Western Blot: B4GALT4 Antibody (5E2) [H00008702-M01] - Analysis of B4GALT4 over-expressed 293 cell line, cotransfected with B4GALT4 Validated Chimera RNAi ( Cat # H00008702-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with B4GALT4 monoclonal antibody (M01), clone 5E2 (Cat # H00008702-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: B4GALT4 Antibody (5E2) [H00008702-M01] - Detection limit for recombinant GST tagged B4GALT4 is approximately 0.1ng/ml as a capture antibody.