Western Blot: RPRD2 Antibody [NB100-367] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-KIAA0460 antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: RPRD2 Antibody [NB100-367] - Whole cell lysate (WCL; amounts indicated) from HeLa cells. Antibody used at 1 ug/ml.
Western Blot: RPRD2 Antibody [NB100-367] - Detection of Human KIAA0460 by Western Blot. Samples: Whole cell lysate (WCL; amounts indicated) from HeLa cells. Antibody: Affinity purified goat anti-KIAA0460 antibody (Cat. No. NB100-367) used at 1 mcg/ml. Detection: Chemiluminescence with 3 minute exposure.
Western Blot: RPRD2 Antibody [NB100-367] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-KIAA0460 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.