Immunohistochemistry: NIF1 Antibody [NB100-2578] - Sample: FFPE section of human thyroid carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-NIF1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunohistochemistry: NIF1 Antibody [NB100-2578] - Sample: FFPE section of human thyroid carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-NIF1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: NIF1 Antibody [NB100-2578] - Detection of Human NIF1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NIF1 antibody NB100-2578 used IP at 3 mcg/mg lysate. NIF1 was also immunoprecipitated by rabbit anti-NIF1 antibodies NB100-2579 and another NIF1 Ab. Detection: Chemiluminescence with an exposure time of 30 seconds.