Western Blot: FIP1/RCP Antibody [NBP2-37698] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Affinity purified rabbit anti-RCP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: FIP1/RCP Antibody [NBP2-37698] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-RCP antibody used for IP at 6 ug per reaction. RCP was also immunoprecipitated by rabbit anti-RCP antibody. For blotting immunoprecipitated RCP, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.