Western Blot: PEPP2 Antibody [NBP2-37696] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Affinity purified rabbit anti-PLEKHA5 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: PEPP2 Antibody [NBP2-37696] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-PLEKHA5 antibody used for IP at 6 ug per reaction. PLEKHA5 was also immunoprecipitated by rabbit anti-PLEKHA5 antibody. For blotting immunoprecipitated PLEKHA5, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.