Western Blot: PTPRCAP Antibody [NBP2-36565] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit PTPRCAP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: PTPRCAP Antibody [NBP2-36565] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. For blotting immunoprecipitated PTPRCAP, it was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.