![Western Blot: ANKHD1 Antibody [NBP1-78211] - Whole cell lysate from 293T (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and HeLa (H; 50 mcg) cells. Affinity purified rabbit anti-ANKHD1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. ANKHD1 was also immunoprecipitated by rabbit anti-ANKHD1 antibody NBP1-78212, which recognizes a downstream epitope.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/29799_ANKHD1-Antibody-Western-Blot-NBP1-78211-img0003.jpg)
Western Blot: ANKHD1 Antibody [NBP1-78211] - Whole cell lysate from 293T (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and HeLa (H; 50 mcg) cells. Affinity purified rabbit anti-ANKHD1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. ANKHD1 was also immunoprecipitated by rabbit anti-ANKHD1 antibody NBP1-78212, which recognizes a downstream epitope.
![Immunoprecipitation: ANKHD1 Antibody [NBP1-78211] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RAP1 antibody NBP2-04097 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. RAP1 was also immunoprecipitated by rabbit anti-RAP1 antibodies NBP2-04098, which recognizes a downstream epitope, and NBP1-78211 which is generated against full-length recombinant human RAP1. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/29800_ANKHD1-Antibody-Immunoprecipitation-NBP1-78211-img0002.jpg)
Immunoprecipitation: ANKHD1 Antibody [NBP1-78211] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RAP1 antibody NBP2-04097 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. RAP1 was also immunoprecipitated by rabbit anti-RAP1 antibodies NBP2-04098, which recognizes a downstream epitope, and NBP1-78211 which is generated against full-length recombinant human RAP1. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).