Western Blot: CARHSP1 Antibody [NBP2-22260] - Whole cell lysate from Jurkat (15 and 50 ug), HeLa (50 ug), and 293T (50 ug) cells. NBP2-22260 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: CARHSP1 Antibody [NBP2-22260] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: NBP2-22260 used for IP at 6 ug/mg lysate. CARHSP1 was also immunoprecipitated by another rabbit anti-CARHSP1 antibody. For blotting immunoprecipitated CARHSP1, NBP2-22260 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.