Western Blot: SECISBP2 Antibody [NBP2-22301] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50ug), and Jurkat (50ug) cells. NBP2-22301 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: SECISBP2 Antibody [NBP2-22301] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22301 used for IP at 6 ug/mg lysate. SBP2 was also immunoprecipitated by rabbit anti-SBP2 antibody. For blotting immunoprecipitated SBP2, NBP2-22301 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.