Western Blot: RAVER1 Antibody [NBP2-22291] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22291 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: RAVER1 Antibody [NBP2-22291] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22291 used for IP at 6 ug/mg lysate. Raver1 was also immunoprecipitated by rabbit anti-Raver1 antibody. For blotting immunoprecipitated Raver1, NBP2-22291 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.