Western Blot: taxilin gamma Antibody [NBP2-22329] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22329 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: taxilin gamma Antibody [NBP2-22329] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22329 used for IP at 6 ug/mg lysate. Gamma-Taxilin was also immunoprecipitated by rabbit anti-Gamma-Taxilin antibodies NBP2-22330 and NBP2-22331. For blotting immunoprecipitated Gamma-Taxilin, NBP2-22329 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.