Western Blot: ABT1 Antibody [NBP2-22332] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22332 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: ABT1 Antibody [NBP2-22332] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22332 used for IP at 6 ug/mg lysate. ABT1 was also immunoprecipitated by rabbit anti-ABT1 antibody. For blotting immunoprecipitated ABT1, NBP2-22332 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.