Immunoprecipitation: Myoferlin Antibody [NBP2-32264] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-Myoferlin antibody NBP2-32264 used for IP at 6 ug per reaction. Myoferlin was also immunoprecipitated by rabbit anti-Myoferlin antibody BL16508. For blotting immunoprecipitated Myoferlin, NBP2-32264 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.