Western Blot: kinectin Antibody [NBP2-32261] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Kinectin antibody NBP2-32261 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: kinectin Antibody [NBP2-32261] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-Kinectin antibody NBP2-32261 used for IP at 6 ug per reaction. For blotting immunoprecipitated Kinectin, NBP2-32261 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.