Western Blot: FAM83G Antibody [NBP2-32191] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-FAM83G antibody NBP2-32191 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: FAM83G Antibody [NBP2-32191] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-FAM83G antibody NBP2-32191 used for IP at 6 ug per reaction. For blotting immunoprecipitated FAM83G, NBP2-32191 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.