Western Blot: ZNF622 Antibody [NBP2-32103] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZPR9 antibody NBP2-32103 used for IP at 6 ug/mg lysate. ZPR9 was also immunoprecipitated by rabbit anti-ZPR9 antibodies A304-075A and A304-076A. For blotting immunoprecipitated ZPR9, NBP2-32103 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: ZNF622 Antibody [NBP2-32103] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-ZPR9 antibody NBP2-32103 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.