![Western Blot: Gamma Adaptin Antibody [NB110-7877] - Analysis shows strong detection of a 91-kDa band corresponding to Human AP1G1 in a HeLa whole cell lysate (lane 1). Peptide competition (using 1 ug/ml of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (lane 2). Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP1G1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature.system was used for signal detection (Roche) using a 60-sec exposure time.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_10/5083_Gamma-Adaptin-Antibody-Western-Blot-NB110-7877-img0002.jpg)
Western Blot: Gamma Adaptin Antibody [NB110-7877] - Analysis shows strong detection of a 91-kDa band corresponding to Human AP1G1 in a HeLa whole cell lysate (lane 1). Peptide competition (using 1 ug/ml of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (lane 2). Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP1G1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature.system was used for signal detection (Roche) using a 60-sec exposure time.