![Western Blot: Notch-1 Antibody (S253-32) [NBP2-22406] - analysis of Rat Brain Membrane showing detection of 270 kDa Notch1 protein using Mouse Anti-Notch1 Monoclonal Antibody, Clone S253-32. Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug).. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Notch1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: 270 kDa.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/23459_Notch-1-Antibody-(S253-32)-Western-Blot-NBP2-22406-img0001.jpg)
Western Blot: Notch-1 Antibody (S253-32) [NBP2-22406] - analysis of Rat Brain Membrane showing detection of 270 kDa Notch1 protein using Mouse Anti-Notch1 Monoclonal Antibody, Clone S253-32. Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug).. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Notch1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: 270 kDa.
![Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (S253-32) [NBP2-22406] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Notch1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/23460_Notch-1%2520Antibody%2520(S253-32)-Immunocytochemistry-Immunofluorescence-NBP2-22406-img0002.jpg)
Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (S253-32) [NBP2-22406] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Notch1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X.