Immunoprecipitation: DHX57 Antibody [NBP1-28693] - Detection of Human DHX57 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DHX57 antibody A302-299A used for IP at 10 mcg/mg lysate. DHX57 was also immunoprecipitated by rabbit anti-DHX57 antibody A302-300A, which recognizes a downstream epitope. For blotting immunoprecipitated DHX57, A302-300A was used at 0.4 mcg/ml, and the ReliaBLOT Reagents and Procedures were incorporated. Detection: Chemiluminescence with an exposure time of 30 seconds.