![Western Blot: DHX57 Antibody [NBP1-28738] - Detection of Human DHX57 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-DHX57 antibody A302-300A used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 10 mcg/mg lysate. DHX57 was also immunoprecipitated by rabbit anti-DHX57 antibody A302-299A, which recognizes an upstream epitope. For blotting immunoprecipitated DHX57, the ReliaBLOT® Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/29634_DHX57%2520Antibody-Western%2520Blot-NBP1-28738-img0001.jpg)
Western Blot: DHX57 Antibody [NBP1-28738] - Detection of Human DHX57 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-DHX57 antibody A302-300A used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 10 mcg/mg lysate. DHX57 was also immunoprecipitated by rabbit anti-DHX57 antibody A302-299A, which recognizes an upstream epitope. For blotting immunoprecipitated DHX57, the ReliaBLOT® Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).