Ancient ubiquitous protein 1 Antibody

• Supplier: Novus Biologicals
• Catalog: NBP1-49916
• Prices: $359.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB IHC IP
• Species Reactivities: Human
• Antigen: The immunogen for this product maps to a region between residue 426 and 476 of human Ancient Ubiquitous Protein 1 using the numbering given in entry NP_853553.1 (GeneID 550).
• Purity/Format: Immunogen affinity purified
• Description: Rabbit Polyclonal
• Gene: AUP1
• Conjugate: Unconjugated

Product images

Western Blot: Ancient ubiquitous protein 1 Antibody [NBP1-49916] - Detection of Human AUP1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-AUP1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. AUP1 was also immunoprecipitated by rabbit anti-AUP1 antibody BL10556, which recognizes an upstream epitope. For blotting immunoprecipitated AUP1 wase used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Immunohistochemistry: Ancient ubiquitous protein 1 Antibody [NBP1-49916] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-AUP1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB