Western Blot: NOL8 Antibody [NBP1-46849] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NOP132 antibody NBP1-46849 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. NOP132 was also immunoprecipitated by rabbit anti-NOP132 antibody NBP1-46850, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Immunohistochemistry: NOL8 Antibody [NBP1-46849] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- NOP132 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB