![Western Blot: Swi6 Antibody [NB600-932] - Analysis is shown using Affinity Purified anti-Swi6 antibody to detect endogenous protein present in S.pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of 43 kDa corresponding to S.pombe Swi6 protein. 35g of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 m nitrocellulose. The blot was incubated with a 1:1,700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_10/5081_Swi6-Antibody-Western-Blot-NB600-932-img0003.jpg)
Western Blot: Swi6 Antibody [NB600-932] - Analysis is shown using Affinity Purified anti-Swi6 antibody to detect endogenous protein present in S.pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of 43 kDa corresponding to S.pombe Swi6 protein. 35g of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 m nitrocellulose. The blot was incubated with a 1:1,700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min.
![Western Blot: Swi6 Antibody [NB600-932] - Analysis of endogenous protein present in S.pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of 43 kDa corresponding to S.pombe Swi6 protein. 35g of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 m nitrocellulose. The blot was incubated with a 1:1,700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_10/5082_Swi6%2520Antibody-Western%2520Blot-NB600-932-img0004.jpg)
Western Blot: Swi6 Antibody [NB600-932] - Analysis of endogenous protein present in S.pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of 43 kDa corresponding to S.pombe Swi6 protein. 35g of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 m nitrocellulose. The blot was incubated with a 1:1,700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min.