![Western Blot: CCL3/MIP-1 alpha Antibody [NBP2-21645] - Anti-mouse MIP-1a shows detection of recombinant mouse MIP-1a/Ccl3 raised in E.coli. Recombinant truncated protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Mouse MIP-1a/Ccl3. After washing, membrane was probed with secondary antibody Dylight649 Conjugated Anti-Rabbit IgG Antibody diluted 1:20,000 in Blocking Buffer for 30 min. at RT.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_10/13024_CCL3-MIP-1-alpha-Antibody-Western-Blot-NBP2-21645-img0001.jpg)
Western Blot: CCL3/MIP-1 alpha Antibody [NBP2-21645] - Anti-mouse MIP-1a shows detection of recombinant mouse MIP-1a/Ccl3 raised in E.coli. Recombinant truncated protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Mouse MIP-1a/Ccl3. After washing, membrane was probed with secondary antibody Dylight649 Conjugated Anti-Rabbit IgG Antibody diluted 1:20,000 in Blocking Buffer for 30 min. at RT.