Western Blot: EBF3 Antibody [NBP2-16254] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: EBF-3 Antibody [NBP2-16254] - U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EBF3 protein stained by EBF3 antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um
Immunoprecipitation: EBF-3 Antibody [NBP2-16254] - Immunoprecipitation of EBF3 protein from U87-MG whole cell extracts using 5 ug of EBF3 antibody [C1C3] Western blot analysis was performed using EBF3 antibody [C1C3] Easy Blot anti-Rabbit IgG was used as a secondary reagent.