Western Blot: ACOT7 Antibody (1D5) [NBP2-22569] - The 293T cell lysate (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACOT7 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry/Immunofluorescence: ACOT7 Antibody (1D5) [NBP2-22569] - Analysis in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACOT7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).