Immunoprecipitation: L3MBTL3 Antibody [NBP1-47314] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-L3MBTL3 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml. L3MBTL3 was also immunoprecipitated by rabbit anti-L3MBTL3 antibodies and, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes.
Immunoprecipitation: L3MBTL3 Antibody [NBP1-47314] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-L3MBTL3 antibody used for IP at 3 ug/mg lysate. L3MBTL3 was also immunoprecipitated by rabbit anti-L3MBTL3 antibodies and, which recognize other epitopes. For blotting immunoprecipitated L3MBTL3, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.