PathScan ® Signaling Nodes Multiplex IF Kit
| Name | PathScan ® Signaling Nodes Multiplex IF Kit |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 8999 |
| Clonality | Polyclonal |
| Antigen | Monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser473 of human Akt, Thr202/Tyr204 of human p44 MAP kinase, and Ser235/Ser236 of human ribosomal protein S6. |
| Description | Polyclonal |
| Gene | AKT1 |
| Supplier Page | Shop |
Product images
Immunofluorescent analysis of MCF7 cells, serum-starved (left) or insulin-treated (right), using PathScan® Signaling Nodes Multiplex IF Kit. Red = Phospho-Akt (Ser473), green = Phospho-p44/42 (Thr202/Tyr204), and blue pseudocolor = Phospho-S6 (Ser235/236).
Immunofluorescent analysis of insulin-treated MCF7 cells (human breast adenocarcinoma), following pretreatment with kinase specific inhibitors LY294002 (PI3 Kinase Inhibitor) #9901 or U0126 (MEK1/2 Inhibitor) #9903 for the indicated times.