
Immunoprecipitation of Atg5 from PANC-1 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Atg5 (D6M7Q) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Atg5 (D6M7Q) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody to avoid cross-reactivity with rabbit IgG.

Confocal immunofluorescent analysis of HCT 116 cells, untreated (left), nutrient starved with EBSS (4 hr, middle), or chloroquine-treated (50 μM, overnight; right) using Atg5 (D6M7Q) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).