Tri-Methyl-Histone H3 (Lys9) Antibody
| Name | Tri-Methyl-Histone H3 (Lys9) Antibody |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 9754 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | WB ICC/IF ChIP |
| Species Reactivities | Human, Mouse, Rat, Monkey, Drosophila, Pig, Horse |
| Antigen | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H3 in which Lys9 is tri-methylated |
| Description | Rabbit Polyclonal |
| Gene | HIST1H3A |
| Supplier Page | Shop |
Product images
Western blot analysis of extracts from various cell lines using Tri-Methyl-Histone H3 (Lys9) Antibody.
Confocal immunofluorescent analysis of PC-3 cells using Tri-Methyl-Histone H3 (Lys9) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells and either 20 μl of Tri-Methyl-Histone H3 (Lys9) Antibody or 2 μl Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human α Satellite Repeat Primers #4486, and SimpleChIP® Human AFM Intron 1 Primers #5098. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.