![Western Blot: Lactate Dehydrogenase Antibody [HRP] [NB600-863] - Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 ™ secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_5/12509_Lactate%2520Dehydrogenase%2520Antibody%2520%255BHRP%255D-Western%2520Blot-NB600-863-img0003.jpg)
Western Blot: Lactate Dehydrogenase Antibody [HRP] [NB600-863] - Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 ™ secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH.
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase Antibody [HRP] [NB600-863] - Tissue: HeLa cells. Fixation: fixed for 5 min in 1:1 MeTOH:Acetone, blocked with MB-071 (preservative free) for 15 min. Antigen retrieval: not required. Primary antibody: Lactate Dehydrogenase antibody at 1:200 for 1 h at RT. Secondary antibody: DyLight 488 conjugated Streptavidin antibody at 1:10,000 for 30 min at RT. Staining: Lactate Dehydrogenase as green fluorescent signal.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_5/12510_Lactate%2520Dehydrogenase%2520Antibody%2520%255BHRP%255D-Immunocytochemistry-Immunofluorescence-NB600-863-img0002.jpg)
Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase Antibody [HRP] [NB600-863] - Tissue: HeLa cells. Fixation: fixed for 5 min in 1:1 MeTOH:Acetone, blocked with MB-071 (preservative free) for 15 min. Antigen retrieval: not required. Primary antibody: Lactate Dehydrogenase antibody at 1:200 for 1 h at RT. Secondary antibody: DyLight 488 conjugated Streptavidin antibody at 1:10,000 for 30 min at RT. Staining: Lactate Dehydrogenase as green fluorescent signal.