Glypican 2 was detected in perfusion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse Glypican 2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2355) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to processes in developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.