Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Regulatory T Cells (Tregs) treated with 10 µg/mL Anti-CD3, 5 µg/mL Anti-CD28, 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B), and 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) for 48 hours were stained with Mouse Anti-Human LAP TGF-beta 1 APC-conjugated Monoclonal Antibody (Catalog # FAB2463A) and either (A) Rat Anti-Human LRRC32/GARP Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB6055G) or (B) Rat IgG2A Alexa Fluor 488 Isotype Control (Catalog # IC006G). View our protocol for Staining Membrane-associated Proteins.