Western Blot: Pipecolic acid oxidase Antibody [HRP] [NB100-2021] - antibody was used to detect Sarcosine Oxidase. Samples of ~1 and 0.25 ug of purified Sarcosine Oxidase per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Rockland Goat anti Sarcosine Oxidase at 4 C. Primary antibody was detected with Dylight 649 conjugated Donkey anti Goat and imaged on the Doc imaging system