Confocal immunofluorescent analysis of THP-1 cells, treated with TPA #4174 (80 nM, 16 hr) (top) or treated with TPA followed by LPS (1 μg/ml, 1 hr) (bottom), using TNF-α (D1G2) Rabbit mAb (Alexa Fluor® 647 Conjugate) (IF/Flow Preferred) (pseudocolor blue). Actin filaments were labeled with Alexa Fluor® 488 phalloidin (Green). Red = Propidium Iodide #4087 (PI)/RNase Staining Solution. Panel shows red/green only (top and bottom left), pseudocolor blue only (top and bottom middle), or merged RGB (top and bottom right).
Flow cytometric analysis of human peripheral blood lymphocytes, untreated (left) or treated with TPA #4174 (40 nM, 4 hr), Ionomycin #9995 (2 μM, 4 hr) and Brefeldin A #9972 (1 μg/ml, last 3 hr of treatment) (right), using a CD3 antibody and TNF-α (D1G2) Rabbit mAb (Alexa Fluor ® 647 Conjugate)