Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti PACAP R1/PACAP receptor 1 Polyclonal Antibody, Unconjugated (bs-0198R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 58kD. Observed band size: 58kD.
Mouse brain lysates and Human A549 cells probed with PACAP receptor 1 Polyclonal Antibody, unconjugated (bs-0198R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.