Formalin-fixed and paraffin embedded rat brain labeled with Anti-MOT8/SLC16A2 Polyclonal Antibody, Unconjugated (bs-11434R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse cells probed with Rabbit Anti-MOT8/SLC16A2 Polyclonal Antibody (bs-11434R) at 1:100 for 60 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue).
Rat H9C2 cells probed with Rabbit Anti-MOT8/SLC16A2 Polyclonal Antibody, Unconjugated (bs-11434R) at 3ug for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-FITC)(green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-MOT8/SLC16A2 Polyclonal Antibody, Unconjugated (bs-11434R) at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining.