Lane 1: Mouse spinal cord lysates probed with Anti –ZNF312 Polyclonal Antibody, Unconjugated (bs-12148R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ZNF312 Polyclonal Antibody, Unconjugated (bs-12148R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.