Lane 1:Hela lysates probed with Rabbit Anti-Phospho1 Polyclonal Antibody, Unconjugated (bs-12562R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Phospho1 Polyclonal Antibody, Unconjugated (bs-12562R) at 1:400 overnight at 4°C, followed.