Human A549 and A431 cell lysates probed with GPR110 Polyclonal Antibody, unconjugated (bs-13513R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded human liver carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with GPR110 Polyclonal Antibody, Unconjugated (bs-13513R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Mouse kidney cells probed with \\tGPR110 Polyclonal Antibody, Unconjugated (bs-13513R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-PE) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).